Targeted MS Experiment. Layers of regulation on cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell [Internet]. Abstract In the budding yeast Saccharomyces cerevisiae, transcription factors TFs regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events.
Experimental evidence coupled with quantitative models suggest that a network of interconnected TFs is capable of regulating periodic genes over the cell cycle. Importantly, these dynamical models were built on transcriptomics data and assumed that TF protein levels and activity are directly correlated with mRNA abundance.
To ask whether TF transcripts match protein expression levels as cells progress through the cell cycle, we applied a multiplexed targeted mass spectrometry approach parallel reaction monitoring on synchronized populations of cells. We found that protein expression of many TFs and cell-cycle regulators closely followed their respective mRNA transcript dynamics in cycling wild-type cells.
We found that a number of proteins were no longer periodically degraded in clb mutants compared to wild type, highlighting the importance of post-transcriptional regulation. This comprehensive investigation of cell-cycle regulators reveals that multiple layers of regulation transcription, protein stability, and proteasome targeting affect protein expression dynamics during the cell cycle.
Experiment Description A PRM assay was developed to target 45 yeast cell-cycle proteins and 4 constitutively-expressed controls. Time course samples were analyzed in a random order and interspersed with QC pool samples containing equal mixtures of all samples. Wild-type cells were collected on filters every 7 minutes across 20 time points 2 replicates.
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Genes controlling essential cell-cycle functions in Drosophila melanogaster.
Moreover, the associated with cell apoptosis gene CARD6 was up-regulated by 2. In general, it is well-documented that some anticancer drugs induce cancer cell apoptosis through the activation of the caspase pathway, especially the caspase-8, -9 and -3 The second scored by the number of pathways network from GeneGo These results were further confirmed by using the GeneGo analysis program, as depicted in Figures 1 , 2 and 3 , showing their possible signaling complex interactions.
The noted changes provide information for understanding the cytotoxic action of DMC at the genetic level.
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Gene alterations may be proven as useful biomarkers or targets for the diagnosis and treatment of human lung cancer in the future. However, further studies are necessary in order to expand or append our current knowledge. The third scored by the number of pathways network from GeneGo User Name Password Sign In.
Correspondence to: Jing-Gung Chung, Ph. Previous Section Next Section. View this table: In this window In a new window. Table I.
Gene Expression: Bursting through the cell cycle
Table II. View larger version: In this window In a new window. Figure 1. Figure 2.
- Cell cycle - Wikipedia.
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